CDNA-DERIVED HYPOVIRUS RNA IN TRANSFORMED CHESTNUT BLIGHT FUNGUS IS SPLICED AND TRIMMED OF VECTOR NUCLEOTIDES

被引：16

作者：

CHEN, BS ^{[1
]}

CRAVEN, MG ^{[1
]}

CHOI, GH ^{[1
]}

NUSS, DL ^{[1
]}

机构：

[1] ROCHE INST MOLEC BIOL,ROCHE RES CTR,NUTLEY,NJ 07110

来源：

VIROLOGY | 1994年 / 202卷 / 01期

关键词：

D O I：

10.1006/viro.1994.1360

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Unencapsidated double-stranded viral RNAs belonging to the genus Hypovirus attenuate virulence of the chestnut blight fungus, Cryphonectria parasitica. A full-length cDNA clone of hypovirus CHV1-713 double-stranded RNA was recently shown to be infectious when introduced into the C. parasitica genome by DNA-mediated transformation. In this study, we show that the viral RNA derived from the chromosomally integrated cDNA copy is trimmed of extraneous vector nucleotide sequences. The cDNA-derived viral RNA was also found to contain a 73-bp deletion located within the 5'-noncoding leader sequence as a result of a pre-mRNA splicing event. Implications of these results are discussed in terms of hypovirus RNA replication and anticipated field studies involving engineered hypovirulent C. parasitica strains. (C) 1994 Academic Press, Inc,

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页码：441 / 448

页数：8

相关论文

共 27 条

[1] HYPOVIRULENCE CONVERSION IN ENDOTHIA-PARASITICA [J].

ANAGNOSTAKIS, SL ;

DAY, PR .

PHYTOPATHOLOGY, 1979, 69 (12) :1226-1229

[2] BIOLOGICAL-CONTROL OF CHESTNUT BLIGHT [J].

ANAGNOSTAKIS, SL .

SCIENCE, 1982, 215 (4532) :466-471

[3] MITOTIC STABILITY AND NUCLEAR INHERITANCE OF INTEGRATED VIRAL CDNA IN ENGINEERED HYPOVIRULENT STRAINS OF THE CHESTNUT BLIGHT FUNGUS [J].

CHEN, BS ;

CHOI, GH ;

NUSS, DL .

EMBO JOURNAL, 1993, 12 (08) :2991-2998

[4] HYPOVIRULENCE OF CHESTNUT BLIGHT FUNGUS CONFERRED BY AN INFECTIOUS VIRAL CDNA [J].

CHOI, GH ;

NUSS, DL .

SCIENCE, 1992, 257 (5071) :800-803

[5] THE AUTOCATALYTIC PROTEASE-P-29 ENCODED BY A HYPOVIRULENCE-ASSOCIATED VIRUS OF THE CHESTNUT BLIGHT FUNGUS RESEMBLES THE POTYVIRUS-ENCODED PROTEASE HC-PRO [J].

CHOI, GH ;

PAWLYK, DM ;

NUSS, DL .

VIROLOGY, 1991, 183 (02) :747-752

[6] NUCLEOTIDE-SEQUENCE OF THE GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GENE FROM CRYPHONECTRIA-PARASITICA [J].

CHOI, GH ;

NUSS, DL .

NUCLEIC ACIDS RESEARCH, 1990, 18 (18) :5566-5566

[7] MOLECULAR ANALYSIS OF THE LACCASE GENE FROM THE CHESTNUT BLIGHT FUNGUS AND SELECTIVE SUPPRESSION OF ITS EXPRESSION IN AN ISOGENIC HYPOVIRULENT STRAIN [J].

CHOI, GH ;

LARSON, TG ;

NUSS, DL .

MOLECULAR PLANT-MICROBE INTERACTIONS, 1992, 5 (02) :119-128

[8] COTRANSLATIONAL AUTOPROTEOLYSIS INVOLVED IN GENE-EXPRESSION FROM A DOUBLE-STRANDED-RNA GENETIC ELEMENT ASSOCIATED WITH HYPOVIRULENCE OF THE CHESTNUT BLIGHT FUNGUS [J].

CHOI, GH ;

SHAPIRA, R ;

NUSS, DL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (04) :1167-1171

[9] PAPAIN-LIKE PROTEASE-P-29 AS A SYMPTOM DETERMINANT ENCODED BY A HYPOVIRULENCE-ASSOCIATED VIRUS OF THE CHESTNUT BLIGHT FUNGUS [J].

CRAVEN, MG ;

PAWLYK, DM ;

CHOI, GH ;

NUSS, DL .

JOURNAL OF VIROLOGY, 1993, 67 (11) :6513-6521

[10]

GRENTE J, 1978, P CHESTNUT S