CLONING AND FUNCTIONAL-CHARACTERIZATION OF THE HUMAN VASOACTIVE-INTESTINAL-PEPTIDE (VIP)-2 RECEPTOR

被引：90

作者：

ADAMOU, JE

AIYAR, N

VANHORN, S

ELSHOURBAGY, NA

机构：

[1] SMITHKLINE BEECHAM PHARMACEUT,DEPT MOLEC GENET,KING OF PRUSSIA,PA 19406

[2] SMITHKLINE BEECHAM PHARMACEUT,DEPT PHARMACOL,KING OF PRUSSIA,PA 19406

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 209卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1515

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A full-length clone encoding the human VIP-2 receptor was isolated from a human placenta cDNA Library. The 1317-bp cDNA insert encodes a protein of 438 amino acids which is a member of the seven transmembrane domain G-protein-coupled receptor superfamily. Expression of the human VIP-2 receptor in COS-7 cells confered high affinity binding to [I-125] VIP (IC50 = 0.93 nM), which was displaced by unlabeled PACAP-38 (IC50 = 6.2 nM). VIP and PACAP-38 were equipotent in stimulating accumulation of cAMP in COS-7 cells transfected with the human VIP-2 receptor. Northern blot analysis revealed two VIP-2 receptor mRNAs of 4.6 kb and 2.3 kb in size which were expressed predominantly in the human skeletal muscle and to a lesser extent in the human brain, heart, pancreas and placenta. (C) 1995. Academic Press, Inc.

引用

页码：385 / 392

页数：8

共 24 条

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