PHOSPHOLIPASE-A2, LEUKOTRIENE-C4 AND PROSTAGLANDIN-E2 LEVELS IN AQUEOUS-HUMOR OF GUINEA-PIGS WITH EXPERIMENTAL S-ANTIGEN INDUCED AUTOIMMUNE UVEITIS

Purified S-antigen was used to induce experimental autoimmune uveitis in 42 guinea pigs. 16 animals were used as controls. Footpad immunization with fresh bovine S-antigen in physiologic saline mixed with complete Freunds''s adjuvant induced a clinical disease in 95% of the eyes in test animals. In aqueous humour the increase in phospholipase-A2 and proteins as well as myeloperoxidase measured from iris-ciliary blocks correlated well with the severity of uveitis evaluated by clinical grading. Leukotriene C4 and prostaglandin E2 were only elevated in aqueous humour drawn from eyes showing a mild form of uveitis. Neither leukotriene C4 nor prostaglandin E2 were detected in eyes graded as clinically moderate or severe. In serum samples phospholipase A2, leukotriene C4, prostaglandin E2 and gamma glutamyl transpeptidase were measured. Of these biochemical parameters, only gamma glutamyl transpeptidase was significantly elevated in test animals with experimental autoimmune uveitis. Histological analysis revealed focal mononuclear cell infiltrations in the choroid. Mononuclear as well as polymorphonuclear cell infiltration was seen predominantly in the pars plana region of the ciliary body of test animals with uveitis. Simultaneous destruction of the outer segments of the photoreceptor layer was seen.