PROPERTIES OF THE IRON-SULFUR PROTEINS OF THE BENZENE DIOXYGENASE SYSTEM FROM PSEUDOMONAS-PUTIDA

被引:55
作者
CRUTCHER, SE
GEARY, PJ
机构
关键词
D O I
10.1042/bj1770393
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A purification procedure was developed to stabilize the iron-sulphur proteins of the benzene dioxygenase system from Pseudomonas putida. The intermediate electron-carrying protein has a mol. wt. of 12300 and possesses one (2Fe--2S) cluster, whereas the terminal dioxygenase has a mol.wt. of 215300 and possesses two (2Fe--2S) clusters. The order and stoicheiometry of electron transfer and of the whole system are described.
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页码:393 / 400
页数:8
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