STRUCTURAL CHARACTERIZATION OF A RICE ACTIN GENE

被引:76
作者
MCELROY, D [1 ]
ROTHENBERG, M [1 ]
WU, R [1 ]
机构
[1] CORNELL UNIV,BIOCHEM MOLEC & CELL BIOL SECT,ITHACA,NY 14853
关键词
actin; cDNA sequence; gene structure; rice; transcript mapping;
D O I
10.1007/BF00018557
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated and sequenced a full-length cDNA clone containing information for the rice actin gene RAc1. Transcript terminus mapping and sequence alignment between the RAc1 cDNA clone and a previously isolated RAc1 genomic clone were used to determine the structure of the RAc1 gene. This allowed us to make the first complete structural characterization of a plant actin gene. The analysis revealed the presence of a 5′-noncoding exon, separated by an intron, from the first translated exon of the RAc1 gene. This is one of the few reported cases of a plant gene containing such a 5′-noncoding exon. Sequence comparison between the previously isolated plant actin genes suggests that such an exon may be a common feature of plant actin gene structure. The present study also confirms that the rice actin gene family is composed of at least eight unique members. © 1990 Kluwer Academic Publishers.
引用
收藏
页码:163 / 171
页数:9
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