IDENTIFICATION AND CHARACTERIZATION OF 2 DERMORPHINS FROM SKIN EXTRACTS OF THE AMAZONIAN FROG PHYLLOMEDUSA-BICOLOR

被引：28

作者：

MIGNOGNA, G

SEVERINI, C

SIMMACO, M

NEGRI, L

ERSPAMER, GF

KREIL, G

BARRA, D

机构：

[1] UNIV ROME LA SAPIENZA,DIPARTIMENTO SCI BIOCHIM A ROSSI FANELLI,PIAZZALE ALDO MORO 5,I-00185 ROME,ITALY

[2] CNR,IST NEUROBIOL,I-00156 ROME,ITALY

[3] UNIV ROME LA SAPIENZA,CNR,CTR BIOL MOLEC,I-00185 ROME,ITALY

[4] UNIV ROME LA SAPIENZA,IST FARMACOL MED,I-00185 ROME,ITALY

[5] AUSTRIAN ACAD SCI,INST MOLEC BIOL,A-5020 SALZBURG,AUSTRIA

来源：

FEBS LETTERS | 1992年 / 302卷 / 02期

关键词：

AMPHIBIAN SKIN; DERMORPHIN; DELTORPHIN; OPIOID PEPTIDE;

D O I：

10.1016/0014-5793(92)80427-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Skin extracts of South American hylid frogs of the subfamily Phyllomedusinae contain dermorphins and deltorphins, opioid heptapeptides highly selective for either mu or delta-receptors. In all these peptides, a D-amino acid is present in the second position. The structure of the precursors for Ala-deltorphins was recently deduced from cloned cDNAs derived from skin of Phyllomedusa bicolor (Richter et al. (1990) Proc. Natl. Acad. Sci. USA 87, 4836-4839). From the amino acid sequence of these precursors, the existence of three peptides related to dermorphin could be predicted. From methanol extracts of skin of Ph. bicolor we have isolated two of these peptides, [Lys7]dermorphin-OH and [Trp4,Asn7]dermorphin-OH. The biological activity of these new dermorphins and their amidated counterparts is presented.

引用

页码：151 / 154

页数：4

共 15 条

[1] STRUCTURE AND PHARMACOLOGICAL ACTIONS OF PHYLLOCAERULEIN, A CAERULEIN-LIKE NONAPEPTIDE - ITS OCCURENCE IN EXTRACTS OF SKIN OF PHYLLOMEDUSA SAUVAGEI AND RELATED PHYLLOMEDUSA SPECIES [J].