Several arbuscular endomycorrhizal fungi (Glomus clarum (LPA16), Scutellospora pellucida (LPA20), Glomus sp. (LPA21), Glomus sp (LPA22) and Glomus sp (LPA25)) were tested for plant growth effects and infection development in Queen Tahiti, Smooth Cayenne (clone CY0) and Spanish varieties of micropropagated pineapple growing in an acid soil under growth chamber tropical conditions. Endomycorrhizal plants of all 3 pineapple varieties grew better than non-mycorrhizal plants. However, increase in plant growth was not related to infection development. Screening of different isolates of arbuscular endomycorrhizal fungi showed some specificity of fungi for promoting growth of the different pineapple varieties. Queen Tahiti and Smooth Cayenne pineapple plants associated with Glomus sp (LPA21) grew better than those infected with the other fungi, whilst best growth was obtained for the Spanish variety by inoculating plants with Glomus sp (LPA25). The root/shoot ratio was modified by endomycorrhizal inoculation, infected pineapple plants showing a greater increase in shoot production in comparison to root production.