MESSENGER-RNA PERIODICAL INFRASTRUCTURE COMPLEMENTARY TO THE PROOFREADING SITE IN THE RIBOSOME

被引:37
作者
LAGUNEZOTERO, J
TRIFONOV, EN
机构
[1] Department of Structural Biology, The Weizmann Institute of Science, Rehovot
关键词
D O I
10.1080/07391102.1992.10508662
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Virtually all mRNA sequences carry a 3-base periodical pattern, presumably involved in the translation frame monitoring mechanism (Trifonov, E.N., J. Mol. Biol. 194,643-652, 87). The hidden pattern, 5'-(GHN)n-3'(H representing nonG, N any base), is further refined by extensive computational analysis of mRNA sequences. According to mononucleotide preferences in the three positions of coding triplets, it appears now as 5'-(GHU)n-3'. Dinucleotide frequencies independent of mononucleotides (contrast dinucleotides, 2) generate the motif 5'-(GCU)n-3'. The same motif is found by regarding the expected avoidance of destabilizing base oppositions in hypothetical transient complementary complexes between mRNA and rRNA. This hidden pattern, in its refined consensus form, 5'-(GCU)n-3', is an almost perfect complementary match to a unique site in small subunit rRNA, the universally conserved (3) proofreading loop at position 525 (of E.coli small subunit rRNA): [GRAPHICS] This strongly suggests that the 525 site is a major structural component of the previously proposed frame-keeping mechanism which is based on the in-frame contacts betwen mRNA and three segments of rRNA. Consistent with the original proposition, this site is one of three believed to interact with mRNA.
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页码:455 / 464
页数:10
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