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TRANSFECTION OF HUMAN LYMPHOBLASTOID CELLS WITH HERPES-SIMPLEX VIRAL-DNA

被引：20
作者：
MILLER, G
WERTHEIM, P
WILSON, G
ROBINSON, J
GEELEN, JLM
VANDERNOORDAA, J
VANDEREB, AJ
机构：
[1] STATE UNIV LEIDEN, PHYSIOL CHEM LAB, LEIDEN, NETHERLANDS
[2] YALE UNIV, SCH MED, DEPT PEDIAT & EPIDEMIOL, NEW HAVEN, CT 06520 USA
[3] YALE UNIV, SCH MED, HOWARD HUGHES MED INST LAB, NEW HAVEN, CT 06510 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 02期
关键词：
D O I：
10.1073/pnas.76.2.949
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The 'calcium/dimethyl sulfoxide shock' method of transfection was adapted for use in human lymphoid cell cultures. One microgram of herpes simplex virus type 1 DNA regularly initiated virus replication in four lymphoblastoid cell lines. Per 105 cells exposed to 1 μg of DNA, 0.5 - 5 cells formed an infectious center. The minimal infective dose of DNA was approximately 500 ng.
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页码：949 / 953
页数：5
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