FULL PROTON NMR ASSIGNMENT OF THE REDUCED FORM OF A REPEAT UNIT TETRASACCHARIDE DERIVED BY KERATANASE DIGESTION OF KERATAN SULFATE

被引：15

作者：

HUCKERBY, TN ^{[1
]}

DICKENSON, JM ^{[1
]}

NIEDUSZYNSKI, IA ^{[1
]}

机构：

[1] UNIV LANCASTER, INST ENVIRONM & BIOL SCI, DIV BIOL SCI, LANCASTER LA1 4YQ, ENGLAND

来源：

MAGNETIC RESONANCE IN CHEMISTRY | 1990年 / 28卷 / 09期

关键词：

!sup]1[!/sup]H NMR; Keratan sulphate tetrasaccharide; Keratanase digestion; Structural assignment;

D O I：

10.1002/mrc.1260280910

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The full assignment of the ambient‐temperature high‐field 1H NMR spectrum for the reduced form of a repeat‐unit β‐linked tetrasaccharide derived by keratanase digestion from keratan sulphate, GlcNAc(6‐SO4)13Gal(6‐SO4)14GlcNAc(6‐SO4)13Gal‐ol, was achieved using two‐dimensional COSY and RELAYED‐COSY experiments. The problem of the choice of the measurement temperature for 1H NMR spectra from keratan sulphate oligomers and polymers is discussed. A modified protocol for the preparation of keratanase‐derived oligosaccharides is proposed. Copyright © 1990 John Wiley & Sons, Ltd.

引用

页码：786 / 791

页数：6

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