A NEW DIRECT METHOD FOR DETERMINING SUPEROXIDE-DISMUTASE ACTIVITY BY MEASURING HYDROGEN-PEROXIDE FORMATION

被引：28

作者：

SEGURAAGUILAR, J

机构：

[1] Unit for Biochemical Toxicology, Department of Bichemistry, Wallenberg Laboratory

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1993年 / 86卷 / 01期

关键词：

SUPEROXIDE DISMUTASE; SUPEROXIDE RADICAL; HYDROGEN PEROXIDE;

D O I：

10.1016/0009-2797(93)90112-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new, direct method for determining superoxide dismutase activity is presented in this study. This method is based on measurement of one of the products of the superoxide dismutase reaction, hydrogen peroxide. Hydrogen peroxide is quantitated using a coupled reaction where horseradish peroxidase catalyzes the formation of a fluorescent product, 6,6'-diOH-(1,1'-biphenyl)-3,3'-diacetic acid, from 4-OH-phenylacetic acid and hydrogen peroxide. Substrate for superoxide dismutase is provided by reduction of oxygen during the autoxidation of riboflavin in the presence of UV light. A linear correlation between the amount of superoxide dismutase (200 ng - 6 mug) and of hydrogen peroxide was found with this method.

引用

页码：69 / 78

页数：10

共 22 条

[1] DIRECT DEMONSTRATION OF SUPEROXIDE ANION PRODUCTION DURING OXIDATION OF REDUCED FLAVIN AND OF ITS CATALYTIC DECOMPOSITION BY ERYTHROCUPREIN [J].