THE POTENTIAL OF FLOW CYTOMETRIC ANALYSIS FOR THE CHARACTERIZATION OF HYBRIDOMA CELLS IN SUSPENSION-CULTURES

被引：13

作者：

COCOMARTIN, JM ^{[1
]}

OBERINK, JW ^{[1
]}

VANDERVELDENDEGROOT, TAM ^{[1
]}

BEUVERY, EC ^{[1
]}

机构：

[1] NATL INST PUBLIC HLTH & ENVIRONM PROTECT,3720 BA BILTHOVEN,NETHERLANDS

来源：

CYTOTECHNOLOGY | 1992年 / 8卷 / 01期

关键词：

FLOW CYTOMETRY; HYBRIDOMAS; MONOCLONAL ANTIBODIES; CYTOPLASMIC IGG; MEMBRANE IGG; CELL CYCLE DISTRIBUTION;

D O I：

10.1007/BF02540031

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Flow cytometric (FC) analysis was applied to determine changes at cellular level during the cultivation of hybridoma cell line MN12 in a suspension batch culture. The relative cell size, cytoplasmic and membrane IgG content and the viability were monitored. Besides, the specificity of the cytoplasmic and membrane IgG was ascertained by means of a synthetic peptide containing the antigenic epitope recognized by the antibody. Cell size was found to increase during the exponential growth phase. The viability as determined by FC follows a similar pattern with the viability data obtained by the conventional trypan blue exclusion test. The relative cytoplasmic and membrane IgG contents were high during the exponential growth and low during stationary phase. Measurement of cell cycle distribution and the antibody content in the culture fluid, indicated that the major part of the cytoplasmic IgG is secreted by cells in the G1-phase. It is concluded that flow cytometry is a useful tool to characterize hybridoma cell lines in a suspension batch culture.

引用

页码：65 / 74

页数：10

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