MEASUREMENT OF ROTATIONAL-DYNAMICS BY THE SIMULTANEOUS NONLINEAR-ANALYSIS OF OPTICAL AND EPR DATA

被引：34

作者：

HUSTEDT, EJ ^{[1
]}

COBB, CE ^{[1
]}

BETH, AH ^{[1
]}

BEECHEM, JM ^{[1
]}

机构：

[1] VANDERBILT UNIV,702 LIGHT HALL,NASHVILLE,TN 37232

来源：

BIOPHYSICAL JOURNAL | 1993年 / 64卷 / 03期

关键词：

D O I：

10.1016/S0006-3495(93)81420-6

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In the preceding companion article in this issue, an optical dye and a nitroxide radical were combined in a new dual function probe, 5-SLE. In this report, it is demonstrated that time-resolved optical anisotropy and electron paramagnetic resonance (EPR) data can be combined in a single analysis to measure rotational dynamics. Rigid-limit and rotational diffusion models for simulating nitroxide EPR data have been incorporated into a general non-linear least-squares procedure based on the Marquardt-Levenberg algorithm. Simultaneous fits to simulated time-resolved fluorescence anisoptropy and linear EPR data, together with simultaneous fits to experimental time-resolved phosphorescence anisotropy decays and saturation transfer EPR (ST-EPR) spectra of 5-SLE noncovalently bound to bovine serum albumin (BSA) have been performed. These results demonstrate that data from optical and EPR experiments can be combined and globally fit to a single dynamic model.

引用

页码：614 / 621

页数：8

共 32 条

[1] PROBING CROSS-BRIDGE ANGULAR TRANSITIONS USING MULTIPLE EXTRINSIC REPORTER GROUPS [J].