STRUCTURE OF THE SNAKE SHORT-CHAIN NEUROTOXIN, ERABUTOXIN-C, PRECURSOR GENE

被引:48
作者
FUSE, N
TSUCHIYA, T
NONOMURA, Y
MENEZ, A
TAMIYA, T
机构
[1] SOPHIA UNIV,FAC SCI & TECHNOL,DEPT CHEM,7-1 KIOI CHO,CHIYODA KU,TOKYO 102,JAPAN
[2] UNIV TOKYO,FAC MED,DEPT PHARMACOL,TOKYO 113,JAPAN
[3] CENS,SERV BIOCHIM,F-91190 GIF SUR YVETTE,FRANCE
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 193卷 / 03期
关键词
D O I
10.1111/j.1432-1033.1990.tb19380.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A 2.4‐kb genomic clone, which encodes the precursor of a snake neurotoxin, erabutoxin c, was isolated from the liver of the sea snake, Laticauda semifasciata. The erabutoxin c gene is about 1.2 kb long and consists of three exons and two introns. The first intron is found at the position corresponding to the signal peptide between amino acid residues 18(Leu) and 19(Gly). The second intron is located at the position corresponding to the central loop of the mature toxin, between amino acid residues 33(Arg) and 34(Gly). A TATA box consensus sequence, characteristic of promoter regions, is identified 29–33 nucleotides upstream from the transcription‐initiation site identified by nuclease S1 analysis. The erabutoxin c cDNA nucleotide sequence, deduced from the present work, is compared with the cDNA sequences of erabutoxins a and b reported previously. Replacements are found at three positions, two of which correspond to the amino acid replacements found among the toxins, while the other is silent. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码:629 / 633
页数:5
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