RETINOIC ACID STIMULATES NEURITE OUTGROWTH IN THE AMPHIBIAN SPINAL-CORD

被引:89
作者
HUNTER, K
MADEN, M
SUMMERBELL, D
ERIKSSON, U
HOLDER, N
机构
[1] UNIV LONDON KINGS COLL,DIV BIOMED SCI,ANAT & HUMAN BIOL GRP,STRAND,LONDON WC2R 2LS,ENGLAND
[2] NATL INST MED RES,DIV PHYS BIOCHEM,LONDON NW7 1AA,ENGLAND
[3] LUDWIG INST CANC RES,STOCKHOLM BRANCH,S-10401 STOCKHOLM,SWEDEN
基金
英国惠康基金;
关键词
RETINOL; CELLULAR RETINOLBINDING AND RETINOIC ACID-BINDING PROTEINS; GLIA;
D O I
10.1073/pnas.88.9.3666
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
There is increasing evidence that retinoic acid (RA), a vitamin A metabolite, plays a role in the development of the nervous system. Here we specifically test this notion by examining the effect of RA on neurite outgrowth from explanted segments of the axolotl spinal cord. We show that there is a threshold concentration in the region 0.1-1 nM above which neurite outgrowth is stimulated 4-5 fold. Retinol, by contrast, only stimulated the migration of glial cells from the explants. Using HPLC we demonstrate that RA and retinol are present endogenously in the axolotl spinal cord. In addition, we have identified by immunocytochemistry with antipeptide antibodies the cells of the spinal cord that contain the binding proteins for RA (cellular RA-binding protein; CRABP) and retinol (cellular retinol-binding protein; CRBP). CRABP is found in the axons and CRBP is found in the ependyma and glial cells. These results provide strong evidence for a role for RA in the developing nervous system, and we propose a specific hypothesis involving CRBP, CRABP, retinol, and RA in the control of axon outgrowth in the spinal cord.
引用
收藏
页码:3666 / 3670
页数:5
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