NITROGEN-METABOLISM IN CULTURES OF TABERNAEMONTANA-DIVARICATA

被引：14

作者：

SCHRIPSEMA, J ^{[1
]}

PELTENBURGLOOMAN, A ^{[1
]}

ERKELENS, C ^{[1
]}

VERPOORTE, R ^{[1
]}

机构：

[1] LEIDEN UNIV,GORLAEUS LABS,DEPT CHEM,DIV NUCL MAGNET RESONANCE,2300 RA LEIDEN,NETHERLANDS

来源：

PHYTOCHEMISTRY | 1991年 / 30卷 / 12期

关键词：

TABERNAEMONTANA-DIVARICATA; APOCYNACEAE; 15N LABELING; NITRATE; AMMONIUM; INDOLE ALKALOIDS; AMINO ACIDS;

D O I：

10.1016/0031-9422(91)83443-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cell suspension culture from Tabernaemontana divaricata was fed with N-15-labelled ammonium or nitrate. The incorporation of label in free amino acids, protein amino acids and indole alkaloids was determined. Ammonium was found to be used more extensively than nitrate in the biosynthesis of these compounds. For tryptamine considerably lower labelling percentages were found than for the indole alkaloid O-acetylvallesamine and the amino acids. This indicates a vacuolar pool of tryptamine, formed at the beginning of the culture-period and not available for further alkaloid biosynthesis.

引用

页码：3951 / 3954

页数：4

共 11 条

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