DEPHOSPHORYLATION OF HUMAN P34(CDC2) KINASE ON BOTH THR-14 AND TYR-15 BY HUMAN CDC25B PHOSPHATASE

被引：88

作者：

HONDA, R

OHBA, Y

NAGATA, A

OKAYAMA, H

YASUDA, H

机构：

[1] KANAZAWA UNIV, FAC PHARMACEUT SCI, DIV BIOL, KANAZAWA, ISHIKAWA 920, JAPAN

[2] UNIV TOKYO, FAC MED, DEPT BIOCHEM, TOKYO 113, JAPAN

来源：

FEBS LETTERS | 1993年 / 318卷 / 03期

关键词：

CDC2; KINASE; CDC25; PROTEIN PHOSPHORYLATION; PHOSPHATASE;

D O I：

10.1016/0014-5793(93)80540-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In mammalian cells, p34cdc2 kinase undergoes phosphorylation at threonine- 1 4, tyrosine-15 and threonine- 1 61 in the S and G2 phases of the cell cycle. At the onset of mitosis, the kinase becomes dephosphorylated at threonine-14 and tyrosine-15, resulting in activation. Cdc25 phosphatase has been shown to dephosphorylate tyrosine-15 in vitro, but whether it also does at threonine-14 remains unclear. In this study, we have found that human cdc25B phosphatase dephosphorylates both threonine-14 and tyrosine-15 but not threonine-161.

引用

页码：331 / 334

页数：4

共 16 条

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