A STUDY OF SOME FACTORS AFFECTING AGROBACTERIUM TRANSFORMATION AND PLANT-REGENERATION OF DENDRANTHEMA-GRANDIFLORA TZVELEV (SYN CHRYSANTHEMUM-MORIFOLIUM RAMAT)

被引:33
作者
LOWE, JM
DAVEY, MR
POWER, JB
BLUNDY, KS
机构
[1] UNIV NOTTINGHAM,DEPT LIFE SCI,PLANT GENET MANIPULAT GRP,NOTTINGHAM NG7 2RD,ENGLAND
[2] ADV TECHNOL CAMBRIDGE LTD,CAMBRIDGE CB4 4WA,ENGLAND
关键词
AGROBACTERIUM; DENDRANTHEMA-GRANDIFLORA; TRANSFORMATION;
D O I
10.1007/BF01983231
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In an attempt to develop a system for producing transformed plants from explants of Dendranthema grandiflora, the susceptibility of the cultivar Super White to various wild-type strains of Agrobacterium tumefaciens and A. rhizogenes was investigated. Tumour formation was not a reliable indicator of the ability of a related disarmed strain to mediate transformation. Following inoculation of explants with disarmed Agrobacterium strains, a number of shoots developed on selective media. However, none of these shoots were transformed. By co-cultivating stem internode explants with a mixed inoculum of wild-type and disarmed strains, it was possible to obtain a callus stably transformed with Agrobacterium carrying a disarmed T-DNA. Histological analysis of explants revealed that shoot regeneration initially occurred from the cells of the epidermis and subsequently from the cortex. However, the cells which were susceptible to T-DNA transfer were confined to the vascular tissue.
引用
收藏
页码:171 / 180
页数:10
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