CYTOSOLIC-FREE [CA2+] IN MONONUCLEAR BLOOD-CELLS FROM DEMENTED PATIENTS AND HEALTHY CONTROLS

被引:20
作者
BONDY, B
KLAGES, U
MULLERSPAHN, F
HOCK, C
机构
[1] Psychiatric Clinic, Department of Neurochemistry, University of Munich, Munich
关键词
ALZHEIMERS DISEASE; LYMPHOCYTES; CALCIUM; PHYTOHEMAGGLUTININ;
D O I
10.1007/BF02191578
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
There is increasing evidence that the neurodegenerative processes in Alzheimer's disease (AD) may be related to alterations in calcium homeostasis and that these metabolic changes are not necessarily restricted to the central nervous system. However, previous studies investigating [Ca2+](i) in fibroblasts, lymphoblasts, platelets and lymphocytes of AD patients gave inconclusive results, since increase, decrease and no alteration in [Ca2+](i) were found in AD patients compared with controls. With respect to the importance of establishing altered Ca2+ homeostasis in peripheral cells, we have investigated [Ca2+](i) in circulating mononuclear cells of patients with AD, multi-infarct dementia, age-associated memory impairment and healthy controls. [Ca2+](i) was evaluated using the fluorescent dye fura-2 before and during stimulation with phythaemagglutinin (PHA). In our study we failed to find major differences in resting [Ca2+](i) and in response to stimulation with 25 mu g/ml and 100 mu g/ml PHA in cells of AD patients as compared with all other groups investigated. There was only a tendency towards a decrease in [Ca2+](i) in AD after stimulation with PHA. Thus the pre sent findings suggest that [Ca2+](i) evaluation in mononuclear cells does not have diagnostic value in discriminating AD patients from other demented patients. However, there might be some difference in [Ca2+](i) values between early- and late-onset AD, which could have pathophysiological importance.
引用
收藏
页码:224 / 228
页数:5
相关论文
共 17 条
  • [1] INCREASED CYTOSOLIC FREE CALCIUM IN LYMPHOCYTES OF ALZHEIMER PATIENTS
    ADUNSKY, A
    BARAM, D
    HERSHKOWITZ, M
    MEKORI, YA
    [J]. JOURNAL OF NEUROIMMUNOLOGY, 1991, 33 (02) : 167 - 172
  • [2] RESTING [CA-2+]I AND [CA-2+]I TRANSIENTS ARE SIMILAR IN FIBROBLASTS FROM NORMAL AND ALZHEIMERS DONORS
    BORDEN, LA
    MAXFIELD, FR
    GOLDMAN, JE
    SHELANSKI, ML
    [J]. NEUROBIOLOGY OF AGING, 1992, 13 (01) : 33 - 38
  • [3] BOSMAN GJCGM, 1991, GERONTOLOGY, V37, P95
  • [4] Age-Associated Memory Impairment: Proposed Diagnostic Criteria and Measures of Clinical Change - Report of a National Institute of Mental Health Work Group
    Crook, Thomas
    Bartus, Raymond T.
    Ferris, Steven H.
    Whitehouse, Peter
    Cohen, Gene D.
    Gershon, Samuel
    [J]. DEVELOPMENTAL NEUROPSYCHOLOGY, 1986, 2 (04) : 261 - 276
  • [5] CALCIUM AND THE AGING NERVOUS-SYSTEM
    GIBSON, GE
    PETERSON, C
    [J]. NEUROBIOLOGY OF AGING, 1987, 8 (04) : 329 - 343
  • [6] CYTOSOLIC FREE CALCIUM IN LYMPHOBLASTS FROM YOUNG, AGED AND ALZHEIMER SUBJECTS
    GIBSON, GE
    TORALBARZA, L
    [J]. MECHANISMS OF AGEING AND DEVELOPMENT, 1992, 63 (01) : 1 - 9
  • [7] PLATELET AND ERYTHROCYTE-MEMBRANE CHANGES IN ALZHEIMERS-DISEASE
    HAJIMOHAMMADREZA, I
    BRAMMER, MJ
    EAGGER, S
    BURNS, A
    LEVY, R
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1025 (02) : 208 - 214
  • [8] CHANGES IN CA2+-BINDING PROTEINS IN HUMAN NEURODEGENERATIVE DISORDERS
    HEIZMANN, CW
    BRAUN, K
    [J]. TRENDS IN NEUROSCIENCES, 1992, 15 (07) : 259 - 264
  • [9] INOSITOL PHOSPHATES AND INTRACELLULAR CALCIUM AFTER BRADYKININ STIMULATION IN FIBROBLASTS FROM YOUNG, NORMAL AGED AND ALZHEIMER DONORS
    HUANG, HM
    TORALBARZA, L
    THALER, H
    TOFELGREHL, B
    GIBSON, GE
    [J]. NEUROBIOLOGY OF AGING, 1991, 12 (05) : 469 - 473
  • [10] MATTSON MP, 1991, ALZHEIMERS DISEASE B