CONFORMATIONAL STUDY OF DOLASTATIN-10

被引：15

作者：

ALATTIA, T

ROUX, F

PONCET, J

CAVE, A

JOUIN, P

机构：

[1] LAB MECANISMES MOLEC COMMUN CELLULAIRES,CNRS,UPR 9023,CTR CNRS,INSERM,F-34094 MONTPELLIER,FRANCE

[2] FAC PHARM MONTPELLIER,CTR BIOCHIM STRUCT,CNRS,UMR 9955,INSERM,U414,F-34060 MONTPELLIER,FRANCE

来源：

TETRAHEDRON | 1995年 / 51卷 / 09期

关键词：

D O I：

10.1016/0040-4020(95)00008-V

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The potent antineoplasic pseudopeptide dolastatin 10 isolated from the sea hare Dolabella auricularia was examined by a combination of one and two dimensional NMR techniques (DQF-COSY, HOHAHA, ROESY, HMQC, and HMBC) followed by restrained molecular-dynamics (MD) simulations to elucidate its conformation in solution (DMSO). The study showed that dolastatin 10 exists in two different conformations corresponding to a cis-trans isomerisation of the Dil-Dap amide bond.

引用

收藏

页码：2593 / 2604

页数：12

相关论文

共 20 条

[1] DIFFERENTIAL-EFFECTS OF ACTIVE ISOMERS, SEGMENTS, AND ANALOGS OF DOLASTATIN-10 ON LIGAND INTERACTIONS WITH TUBULIN - CORRELATION WITH CYTOTOXICITY [J].

BAI, R ;

ROACH, MC ;

JAYARAM, SK ;

BARKOCZY, J ;

PETTIT, GR ;

LUDUENA, RF ;

HAMEL, E .

BIOCHEMICAL PHARMACOLOGY, 1993, 45 (07) :1503-1515

[2]

BAI R, 1990, J BIOL CHEM, V265, P17141

[3] MLEV-17-BASED TWO-DIMENSIONAL HOMONUCLEAR MAGNETIZATION TRANSFER SPECTROSCOPY [J].

BAX, A ;

DAVIS, DG .

JOURNAL OF MAGNETIC RESONANCE, 1985, 65 (02) :355-360

[4] H-1 AND C-13 ASSIGNMENTS FROM SENSITIVITY-ENHANCED DETECTION OF HETERONUCLEAR MULTIPLE-BOND CONNECTIVITY BY 2D MULTIPLE QUANTUM NMR [J].

BAX, A ;

SUMMERS, MF .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (08) :2093-2094

[5] SENSITIVITY-ENHANCED TWO-DIMENSIONAL HETERONUCLEAR SHIFT CORRELATION NMR-SPECTROSCOPY [J].

BAX, A ;

SUBRAMANIAN, S .

JOURNAL OF MAGNETIC RESONANCE, 1986, 67 (03) :565-569

[6] PRACTICAL ASPECTS OF TWO-DIMENSIONAL TRANSVERSE NOE SPECTROSCOPY [J].

BAX, A ;

DAVIS, DG .

JOURNAL OF MAGNETIC RESONANCE, 1985, 63 (01) :207-213

[7] RAPID-PULSING ARTIFACTS IN DOUBLE-QUANTUM-FILTERED COSY [J].

DEROME, AE ;

WILLIAMSON, MP .

JOURNAL OF MAGNETIC RESONANCE, 1990, 88 (01) :177-185

[8] SYNTHESIS OF THE CYCLODEPSIPEPTIDE NORDIDEMNIN-B, A CYTO-TOXIC MINOR PRODUCT ISOLATED FROM THE SEA TUNICATE TRIDIDEMNUM-CYANOPHORUM [J].

JOUIN, P ;

PONCET, J ;

DUFOUR, MN ;

PANTALONI, A ;

CASTRO, B .

JOURNAL OF ORGANIC CHEMISTRY, 1989, 54 (03) :617-627

[9] NUCLEAR MAGNETIC-RESONANCE ANALYSES OF SIDE-CHAIN CONFORMATIONS OF HISTIDINE AND AROMATIC AMINO-ACID DERIVATIVES - SOLVENT AND PH-DEPENDENCE [J].

KOBAYASHI, JI ;

HIGASHIJIMA, T ;

MIYAZAWA, T .

INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 1984, 24 (01) :40-47

[10] NUCLEAR MAGNETIC RESONANCE STUDY OF SOME ALPHA-AMINO ACIDS .2. ROTATIONAL ISOMERISM [J].

PACHLER, KGR .

SPECTROCHIMICA ACTA, 1964, 20 (04) :581-587