ESCHERICHIA-COLI MUTY-DEPENDENT MISMATCH REPAIR INVOLVES DNA-POLYMERASE-I AND A SHORT REPAIR TRACT

被引：11

作者：

TSAIWU, JJ ^{[1
]}

LU, AL ^{[1
]}

机构：

[1] UNIV MARYLAND,SCH MED,DEPT BIOL CHEM,BALTIMORE,MD 21201

来源：

MOLECULAR & GENERAL GENETICS | 1994年 / 244卷 / 04期

关键词：

ESCHERICHIA COLI; MUTY; DNA MISMATCH REPAIR; DNA POLYMERASE; REPAIR TRACT;

D O I：

10.1007/BF00286698

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Repair of heteroduplex DNA containing an A/G mismatch in a mutL background requires the Escherichia coli mutY gene function. The mutY-dependent in vitro repair of A/G mismatches is accompanied by repair DNA synthesis on the DNA strand bearing mispaired adenines. The size of the mutY-dependent repair tract was measured by the specific incorporation of alpha[P-32]dCTP into different restriction fragments of the repaired DNA. The repair tract is shorter than 12 nucleotides and longer than 5 nucleotides and is localized to the 3' side of the mismatched adenine. This repair synthesis is carried out by DNA polymerase I.

引用

页码：444 / 450

页数：7

共 45 条

[1] ESCHERICHIA-COLI MUTY GENE-PRODUCT IS REQUIRED FOR SPECIFIC A-G-]C.G MISMATCH CORRECTION [J].

AU, KG ;

CABRERA, M ;

MILLER, JH ;

MODRICH, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :9163-9166

[2] ESCHERICHIA-COLI MUTY GENE ENCODES AN ADENINE GLYCOSYLASE ACTIVE ON G-A MISPAIRS [J].

AU, KG ;

CLARK, S ;

MILLER, JH ;

MODRICH, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) :8877-8881

[3]

AU KG, 1992, J BIOL CHEM, V267, P12142

[4] ESCHERICHIA-COLI ENDONUCLEASE-III IS NOT AN ENDONUCLEASE BUT A BETA-ELIMINATION CATALYST [J].

BAILLY, V ;

VERLY, WG .

BIOCHEMICAL JOURNAL, 1987, 242 (02) :565-572

[5] ASYMMETRIC REPAIR OF BACTERIOPHAGE-T7 HETERODUPLEX DNA [J].

BAUER, J ;

KRAMMER, G ;

KNIPPERS, R .

MOLECULAR AND GENERAL GENETICS, 1981, 181 (04) :541-547

[6]

BREIMER LH, 1984, J BIOL CHEM, V259, P5543

[7] D(GATC) SEQUENCES INFLUENCE ESCHERICHIA-COLI MISMATCH REPAIR IN A DISTANCE-DEPENDENT MANNER FROM POSITIONS BOTH UPSTREAM AND DOWNSTREAM OF THE MISMATCH [J].

BRUNI, R ;

MARTIN, D ;

JIRICNY, J .

NUCLEIC ACIDS RESEARCH, 1988, 16 (11) :4875-4890

[8] DNA N-GLYCOSYLASES AND UV REPAIR [J].

DEMPLE, B ;

LINN, S .

NATURE, 1980, 287 (5779) :203-208

[9] GENETIC REQUIREMENTS FOR HYPER-RECOMBINATION BY VERY SHORT PATCH MISMATCH REPAIR - INVOLVEMENT OF ESCHERICHIA-COLI DNA-POLYMERASE-I [J].

DZIDIC, S ;

RADMAN, M .

MOLECULAR & GENERAL GENETICS, 1989, 217 (2-3) :254-256

[10]

Friedberg E. C., 1985, DNA REPAIR

← 1 2 3 4 5 →