A NOVEL-APPROACH TO INSERTIONAL MUTAGENESIS OF HAEMOPHILUS-INFLUENZAE

被引：34

作者：

SHARETZSKY, C

EDLIND, TD

LIPUMA, JJ

STULL, TL

机构：

[1] MED COLL PENN,DEPT PEDIAT,PHILADELPHIA,PA 19129

[2] MED COLL PENN,DEPT MICROBIOL IMMUNOL,PHILADELPHIA,PA 19129

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 04期

关键词：

D O I：

10.1128/jb.173.4.1561-1564.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Insertional mutagenesis of the Haemophilus influenzae chromosome was accomplished by a novel method employing a 2.2-kbp element, TSTE. This element, consisting of the neo gene of Tn5 flanked by Haemophilus-specific uptake sequences, was ligated to circularized chromosomal fragments before transformation into the homologous strain. Eight mutants defective in the production of haemocin were detected. This strategy provides an efficient mechanism for the insertional mutagenesis of H. influenzae.

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页码：1561 / 1564

页数：4

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