A 16S RIBOSOMAL-RNA TARGETED OLIGONUCLEOTIDE PROBE FOR THE DIFFERENTIATION OF STAPHYLOCOCCUS-HAEMOLYTICUS FROM S WARNERI AND S HOMINIS

被引：5

作者：

DRUEL, B

WEIZENEGGER, M

LUDWIG, W

FRENEY, J

SCHLEIFER, KH

FLEURETTE, J

机构：

[1] HOP EDOUARD HERRIOT,MICROBIOL LAB,F-69437 LYON 03,FRANCE

[2] CTR NATL REFERENCE STAPHYLOCOQUES,F-69437 LYON 03,FRANCE

[3] TECH UNIV MUNICH,LEHRSTUHL MIKROBIOL,W-8000 MUNICH 2,GERMANY

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 1992年 / 15卷 / 04期

关键词：

COAGULASE-NEGATIVE STAPHYLOCOCCI; 16S RIBOSOMAL-RNA; OLIGONUCLEOTIDE PROBE;

D O I：

10.1016/S0723-2020(11)80113-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sequencing and comparison of 16S rRNA genes of Staphylococcus haemolyticus, S. hominis and S. carnosus allowed the development of an oligonucleotide probe that is complementary to a region of S. haemolyticus 16S rRNA and allows the differentiation of this species from the other two. After hybridization with 27 staphylococcal reference strains and 63 clinical strains, we found this probe useful as an alternative to the ID 32 Staph gallery (bioMerieux) in the case of an identification problem between S. haemolyticus and S. hominis or S. warneri. The oligonucleotide probe was used for the characterization of two misidentified strains.

引用

页码：535 / 537

页数：3

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