A PROTEIN-SPECIFIC MONOCLONAL-ANTIBODY TO RAT-LIVER BETA-1-]4 GALACTOSYLTRANSFERASE AND ITS APPLICATION TO IMMUNOHISTOCHEMISTRY

被引：39

作者：

KAWANO, JI ^{[1
]}

IDE, S ^{[1
]}

OINUMA, T ^{[1
]}

SUGANUMA, T ^{[1
]}

机构：

[1] MIYAZAKI MED COLL, DEPT ANAT, MIYAZAKI 88916, JAPAN

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1994年 / 42卷 / 03期

关键词：

GALACTOSYLTRANSFERASE; RAT; LIVER; EPIDIDYMIS; PAROTID GLAND; SUBMANDIBULAR GLAND; SUBLINGUAL GLAND; GOLGI APPARATUS; MONOCLONAL ANTIBODY; IMMUNOHISTOCHEMISTRY;

D O I：

10.1177/42.3.8308253

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have produced a new protein-specific monoclonal antibody (MAb) to rat liver beta 1 --> 4 galactosyltransferase. This MAb, GTL2, was selected as the most reactive IgG to a periodate-treated antigen. Antigen and protein specificities of GTL2 were verified by immunoblotting of a non-glycosylated recombinant protein of human galactosyltransferase and enzymatically deglycosylated rat galactosyltransferase. Using GTL2, an immunohistochemical study was done in rat liver, epididymis, and salivary glands. Intense staining was observed in Golgi areas of epididymal duct epithelial cells, and submandibular and sublingual acinar cells. Hepatocytes showed weaker staining. Immunoelectron microscopic observation revealed that the staining was exclusively localized in trans-Golgi membranes of these cells.

引用

页码：363 / 369

页数：7

共 35 条

[1]

AMANO J, 1991, J BIOL CHEM, V266, P11461

[2] LIGHT MICROSCOPIC LOCALIZATION OF GLYCOSYLTRANSFERASE ACTIVITIES IN CELLS AND TISSUES [J].