CLONING, EXPRESSION AND CHARACTERIZATION OF HUMAN THIOLTRANSFERASE (GLUTAREDOXIN) IN ESCHERICHIA-COLI

被引：42

作者：

CHRESTENSEN, CA

ECKMAN, CB

STARKE, DW

MIEYAL, JJ

机构：

[1] CASE WESTERN RESERVE UNIV, SCH MED, DEPT PHARMACOL, CLEVELAND, OH 44106 USA

[2] CASE WESTERN RESERVE UNIV, SCH MED, DEPT NEUROSCI, CLEVELAND, OH 44106 USA

来源：

FEBS LETTERS | 1995年 / 374卷 / 01期

关键词：

THIOLTRANSFERASE; GLUTAREDOXIN; CLONING; SULFHYDRYL;

D O I：

10.1016/0014-5793(95)01066-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

PCR primers were designed from the known amino acid (aa) sequence for human red blood cell thioltransferase (hRBC TTase) and the known cDNA sequence for pig liver TTase (82% homologous) and used to amplify thioltransferase from a pool of human brain cDNAs. The PCR product was inserted into the pKK233-2 expression vector. The DNA sequence of the insert agreed with the aa sequence. High level expression of the enzyme was accomplished in E. coli, and Western blot analysis confirmed its identity. Recombinant TTase displayed catalytic properties indistinguishable from natural hRBC TTase.

引用

页码：25 / 28

页数：4

共 20 条

[1]

PROTEIN S-THIOLATION IN HEPATOCYTES STIMULATED BY T-BUTYL HYDROPEROXIDE, MENADIONE, AND NEUTROPHILS [J].