NUCLEOSIDE PHOSPHOTRANSFERASE FROM YELLOW LUPIN SEEDLING COTYLEDONS

被引:19
作者
GURANOWSKI, A
机构
[1] Institute of Biochemistry, Academy of Agriculture, PL-60-637 Poznań
关键词
(Lupin cotyledon); Nucleoside phosphotransferase;
D O I
10.1016/0005-2744(79)90076-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nucleoside phosphotransferase (nucleotide:3′-deoxynucleoside 5′-phosphotransferase, EC 2.7.1.77) from yellow lupin seedling cotyledons was purified and the active enzyme consists of a single polypeptide chain, Mr = 72 000 ± 3000. In transphosphorylation, purine and pyrimidine nucleosides are good phosphate acceptors and 5′-nucleotides are effective phosphate donors. Among 2′- and 3′-nucleotides, only ‴t-AMP and 3′-ψMP acted as phosphate donors, and p-nitrophenylphosphate appeared less active in this regard. The purine and pyrimidine bases inhibit transphosphorylation. The Km values determined for the inosine:5′-AMP pair were 400 μM for both the compounds. The enzyme showed optimum activity at pH 8.0 in 50 mM Tris-HCl buffer. Antisulfhydryl reagents and EDTA did not affect enzyme activity. © 1979.
引用
收藏
页码:13 / 22
页数:10
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