CHARACTERIZATION OF REACTIONS CATALYZED BY YEAST PHOSPHATIDYLINOSITOL SYNTHASE

被引：15

作者：

KLEZOVITCH, O ^{[1
]}

BRANDENBURGER, Y ^{[1
]}

GEINDRE, M ^{[1
]}

DESHUSSES, J ^{[1
]}

机构：

[1] UNIV GENEVA,DEPT BIOCHEM,30 QUAI E ANSERMET,CH-1211 GENEVA 4,SWITZERLAND

来源：

FEBS LETTERS | 1993年 / 320卷 / 03期

关键词：

CDP-DIACYLGLYCEROL; PHOSPHATIDYLINOSITOL SYNTHASE; EXCHANGE REACTION; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1016/0014-5793(93)80598-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nature of reactions catalysed by yeast phosphatidylinositol synthase expressed in E. coli has been investigated. The single enzyme is shown to carry both CDP-diacylglycerol-dependent incorporation of inositol into phosphatidylinositol (K(m) for inositol of 0.090 mM) and a CDP-diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol (K(m) for inositol of 0.066 mM). The exchange reaction and reversal of phosphatidylinositol synthase were both stimulated by CMP, but had different optimum pH and requirements for substrates. These results suggest that CMP-stimulated exchange and CMP-dependent reverse reactions are distinct processes catalysed by the same enzyme, phosphatidylinositol synthase.

引用

页码：256 / 260

页数：5

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