BINDING OF HISTONE H1 TO DNA IS INDIFFERENT TO METHYLATION AT CPG SEQUENCES

被引：38

作者：

CAMPOY, FJ ^{[1
]}

MEEHAN, RR ^{[1
]}

MCKAY, S ^{[1
]}

NIXON, J ^{[1
]}

BIRD, A ^{[1
]}

机构：

[1] UNIV EDINBURGH,INST CELL & MOLEC BIOL,EDINBURGH EH9 3JR,MIDLOTHIAN,SCOTLAND

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 44期

基金：

英国惠康基金;

关键词：

D O I：

10.1074/jbc.270.44.26473

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The possibility that histone H1 binds preferentially to DNA containing 5-methylcytosine in the dinucleotide CpG is appealing, as it could help to explain the repressive effects of methylation on gene activity, In this study, the affinity of purified H1 for methylated and non-methylated DNA sequences has been tested using both naked DNA and chromatin, Based on a variety of assays (bandshifts, filter-binding assays, Southwestern blots, and nuclease sensitivity assays), we conclude that H1 has no significant preference for binding to naked methylated DNA, Similarly, H1 showed the same affinities for methylated and non-methylated DNA when assembled into chromatin in a Xenopus oocyte extract. Thus potential cooperative interaction of H1 with polynucleosomal complexes is not enhanced by the presence of DNA methylation.

引用

页码：26473 / 26481

页数：9

共 43 条

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