DISRUPTION OF A GENE ENCODING A NOVEL THIOREDOXIN-LIKE PROTEIN ALTERS THE CYANOBACTERIAL PHOTOSYNTHETIC APPARATUS

被引:7
作者
COLLIER, JL
GROSSMAN, AR
机构
[1] CARNEGIE INST WASHINGTON,DEPT PLANT BIOL,STANFORD,CA 94305
[2] UNIV CALIF SAN DIEGO,SCRIPPS INST OCEANOG,DIV BIOMED MARINE RES,LA JOLLA,CA 92093
关键词
D O I
10.1128/jb.177.11.3269-3276.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A gene that may encode a novel protein disulfide oxidoreductase, designated txlA (thioredodn-like), was isolated from the cyanobacterium Synechococcus sp. strain PCC7942. Interruption of txlA near the putative thioredoxin-like active site yielded cells that grew too poorly to be analyzed. In contrast, a disruption of txlA near the C terminus that left the thioredoxin-like domain intact yielded two different mutant phenotypes. One type, designated txlXb, exhibited a slightly reduced growth rate and an increased cellular content of apparently normal phycobilisomes. The cellular content of phycobilisomes also increased in the other mutant strain, designated txlXg. However, txlXg also exhibited a proportionate increase in chlorophyll and other components of the photosynthetic apparatus and grew as fast as wild-type cells. Both the txlXb and txlXg phenotypes were stable. The differences between the two strains may result from a genetic polymorphism extant in the original cell population. Further mayestigation of txlA may provide new insights into mechanisms that regulate the structure and function of the cyanobacterial photosynthetic apparatus.
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收藏
页码:3269 / 3276
页数:8
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