Experimental NMR techniques for studies of biopolymers

被引：10

作者：

Bax, Ad ^{[1
]}

机构：

[1] NIH, Bethesda, MD USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1991年 / 1卷 / 06期

关键词：

D O I：

10.1016/0959-440X(91)90102-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NMR technology has advanced significantly during the past year. Four-dimensional NMR spectroscopy is a feasible approach for unraveling the extremely crowded NOE spectra of medium-sized proteins. Alternatively, the use of selective deuteration also shows promise in this area. New three-dimensional NMR techniques can be used for accurate measurement of multiple-bond J couplings and improved methods are appearing for measurement of heteronuclear relaxation times, providing a more accurate characterization of internal protein dynamics.

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页码：1030 / 1035

页数：6

相关论文

共 40 条

[1] SEQUENCE-SPECIFIC H-1-NMR ASSIGNMENTS AND SECONDARY STRUCTURE IN SOLUTION OF ESCHERICHIA-COLI TRP REPRESSOR [J].

ARROWSMITH, CH ;

PACHTER, R ;

ALTMAN, RB ;

IYER, SB ;

JARDETZKY, O .

BIOCHEMISTRY, 1990, 29 (27) :6332-6341

[2]

BAX A, 1991, Journal of Biomolecular NMR, V1, P99, DOI 10.1007/BF01874573

[3] H-1-H-1 CORRELATION VIA ISOTROPIC MIXING OF C-13 MAGNETIZATION, A NEW 3-DIMENSIONAL APPROACH FOR ASSIGNING H-1 AND C-13 SPECTRA OF C-13-ENRICHED PROTEINS [J].

BAX, A ;

CLORE, GM ;

GRONENBORN, AM .

JOURNAL OF MAGNETIC RESONANCE, 1990, 88 (02) :425-431

[4] PRACTICAL ASPECTS OF PROTON CARBON CARBON PROTON 3-DIMENSIONAL CORRELATION SPECTROSCOPY OF C-13-LABELED PROTEINS [J].

BAX, A ;

CLORE, GM ;

DRISCOLL, PC ;

GRONENBORN, AM ;

IKURA, M ;

KAY, LE .

JOURNAL OF MAGNETIC RESONANCE, 1990, 87 (03) :620-627

[5] OBSERVATION OF SPIN DIFFUSION IN BIOMOLECULES BY 3-DIMENSIONAL NOE-NOE SPECTROSCOPY [J].

BOELENS, R ;

VUISTER, GW ;

KONING, TMG ;

KAPTEIN, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (22) :8525-8526

[6] INFLUENCE OF CROSS-CORRELATION BETWEEN DIPOLAR AND ANISOTROPIC CHEMICAL-SHIFT RELAXATION MECHANISMS UPON LONGITUDINAL RELAXATION RATES OF N-15 IN MACROMOLECULES [J].

BOYD, J ;

HOMMEL, U ;

CAMPBELL, ID .

CHEMICAL PHYSICS LETTERS, 1990, 175 (05) :477-482

[7] 3D NOE-NOE SPECTROSCOPY OF PROTEINS - OBSERVATION OF SEQUENTIAL 3D NOE CROSS PEAKS IN ARC REPRESSOR [J].

BREG, JN ;

BOELENS, R ;

VUISTER, GW ;

KAPTEIN, R .

JOURNAL OF MAGNETIC RESONANCE, 1990, 87 (03) :646-651

[8] MEASUREMENT OF SMALL HETERONUCLEAR H-1-N-15 COUPLING-CONSTANTS IN N-15-LABELED PROTEINS BY 3D HNNHAB-COSY [J].

CHARY, KVR ;

OTTING, G ;

WUTHRICH, K .

JOURNAL OF MAGNETIC RESONANCE, 1991, 93 (01) :218-224

[9]

CLARE GM, 1991, J BIOMOL NMR, V1, P13

[10] APPLICATIONS OF 3-DIMENSIONAL AND 4-DIMENSIONAL HETERONUCLEAR NMR-SPECTROSCOPY TO PROTEIN-STRUCTURE DETERMINATION [J].

CLORE, GM ;

GRONENBORN, AM .

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 1991, 23 :43-92

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