MULTIPLICATION AND DIFFERENTIATION OF TRYPANOSOMA CRUZI IN AN INSECT CELL CULTURE SYSTEM

被引:24
作者
WOOD, DE
PIPKIN, AC
机构
[1] Department of Parasitology, Naval Medical Research Institute, Bethesda, MD
关键词
Culture; Life history; Trypanosoma cruzi;
D O I
10.1016/0014-4894(69)90155-6
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Trypanosoma cruzi was successfully cultured in an insect cell culture system. Transformation of trypomastigote forms from infected mouse blood into dividing amastigote forms was observed. Differentiation of the amastigote forms into large and predictable numbers of trypomastigote forms occurred in this system. Parasite populations of 49 ± 429 × 106 organisms/ml of culture and a population doubling time of 14 hours have been achieved. © 1969.
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页码:176 / &
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