ALTERATIONS TO THE CATALYTIC PROPERTIES OF POLYPHENOLOXIDASE IN DETERGENTLESS MICROEMULSIONS AND TERNARY WATER ORGANIC-SOLVENT MIXTURES

被引：19

作者：

VULFSON, EN ^{[1
]}

AHMED, G ^{[1
]}

GILL, I ^{[1
]}

KOZLOV, IA ^{[1
]}

GOODENOUGH, PW ^{[1
]}

LAW, BA ^{[1
]}

机构：

[1] UNIV READING, DEPT FOOD TECHNOL, READING RG6 2AH, BERKS, ENGLAND

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 02期

关键词：

D O I：

10.1007/BF01030457

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The catalytic properties of mushroom polyphenoloxidase could be substantially altered by entrapment into hexane- and toluene-based microemulsions stabilized with isopropanol. The fast irreversible inactivation and drastic substrate inhibition of the enzyme were significantly reduced in detergentless microemulsions in comparison to conventional aqueous media. Similar changes in the catalytic behavior of polyphenoloxidase were observed in the normal ternary solutions of hexane-(toluene)-isopropanol-water, and in the H-bonded aggregates of isopropanol and water in toluene, but not in hexane.

引用

页码：91 / 96

页数：6