LOCALIZATION AND POLYMORPHISM OF A CHROMOSOME 12-SPECIFIC ALPHA-SATELLITE DNA-SEQUENCE

被引：73

作者：

LOOIJENGA, LHJ

SMIT, VTHBM

WESSELS, JW

MOLLEVANGER, P

OOSTERHUIS, JW

CORNELISSE, CJ

DEVILEE, P

机构：

[1] LEIDEN STATE UNIV,DEPT HUMAN GENET,WASSENAARSEWEG 72,POSTBUS 9503,2312 AV LEIDEN,NETHERLANDS

[2] LEIDEN STATE UNIV,DEPT PATHOL,2312 AV LEIDEN,NETHERLANDS

[3] STATE UNIV GRONINGEN,DEPT PATHOL,9700 AB GRONINGEN,NETHERLANDS

来源：

CYTOGENETICS AND CELL GENETICS | 1990年 / 53卷 / 04期

关键词：

D O I：

10.1159/000132934

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The isolation and localization of a chromosome 12-specific a satellite DNA sequence. pul2H8. is described. This clone contains a complete copy of the l.4-kb//imJIU higher-order repeat present within the α satellite array on chromosome 12. The specificity of pul2H8 was demonstrated by in situ hybridization mid Southern blot analysis of a somatic cell hybrid mapping panel, both performed under high-stringency conditions Polymorphic restriction patterns within the a satellite array, revealed by the use of (he restriction enzymes Bg IH and EcoRV, were demonstrated to display Mendelian inheritance. These properties make pα12H8 a valuable genetic marker for I he centromeric region of chromosome 12. © 1990 S. Karger AG, Basel.

引用

页码：216 / &

共 12 条

[1]

BALDINI A, 1990, AM J HUM GENET, V46, P784

[2]

CASTEDO SMMJ, 1989, CANCER RES, V49, P5696

[3] DETECTION OF CHROMOSOME-ABERRATIONS IN THE HUMAN INTERPHASE NUCLEUS BY VISUALIZATION OF SPECIFIC TARGET DNAS WITH RADIOACTIVE AND NONRADIOACTIVE INSITU HYBRIDIZATION TECHNIQUES - DIAGNOSIS OF TRISOMY-18 WITH PROBE L1.84 [J].