A TRANSFERABLE PLASMID ASSOCIATED WITH AS-48 PRODUCTION IN ENTEROCOCCUS-FAECALIS

被引：72

作者：

MARTINEZBUENO, M ^{[1
]}

GALVEZ, A ^{[1
]}

VALDIVIA, E ^{[1
]}

MAQUEDA, M ^{[1
]}

机构：

[1] UNIV GRANADA,FAC CIENCIAS,DEPT MICROBIOL,E-18071 GRANADA,SPAIN

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 05期

关键词：

D O I：

10.1128/jb.172.5.2817-2818.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enterococcus faecalis S-48 produces a peptide antibiotic, AS-48, and a bacteriocin, Bc-48. We have isolated mutants that lack these inhibitory characteristics. Further analysis of the mutants indicates that a plasmid of 56 kilobases (pMB2) may harbor the genes for AS-48. In conjugation experiments, pMB2 has been transferred into a plasmid-free OG1X strain of E. faecalis. The OG1X(pMB2) transconjugant produces the antibiotic AS-48 in solid medium, and the MIC of AS-48 for this strain is the same as that of the donor strain.

引用

页码：2817 / 2818

页数：2

共 11 条

[1] SIMPLE AND RAPID METHOD FOR ISOLATING LARGE PLASMID DNA FROM LACTIC STREPTOCOCCI [J].

ANDERSON, DG ;

MCKAY, LL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 46 (03) :549-552

[2] BACTERICIDAL AND BACTERIOLYTIC ACTION OF PEPTIDE ANTIBIOTIC AS-48 AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA AND OTHER ORGANISMS [J].

GALVEZ, A ;

MAQUEDA, M ;

MARTINEZBUENO, M ;

VALDIVIA, E .

RESEARCH IN MICROBIOLOGY, 1989, 140 (01) :57-68

[3] PURIFICATION AND AMINO-ACID COMPOSITION OF PEPTIDE ANTIBIOTIC AS-48 PRODUCED BY STREPTOCOCCUS-(ENTEROCOCCUS)-FAECALIS SUBSP LIQUEFACIENS S-48 [J].

GALVEZ, A ;

GIMENEZGALLEGO, G ;

MAQUEDA, M ;

VALDIVIA, E .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1989, 33 (04) :437-441

[4] EFFECT OF PEPTIDE AS-48 ON ENTEROCOCCUS-FAECALIS SUBSP LIQUEFACIENS S-47 [J].

GALVEZ, A ;

VALDIVIA, E ;

MARTINEZ, M ;

MAQUEDA, M .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1989, 33 (05) :641-645

[5] CHARACTERIZATION AND PARTIAL-PURIFICATION OF A BROAD-SPECTRUM ANTIBIOTIC AS-48 PRODUCED BY STREPTOCOCCUS-FAECALIS [J].

GALVEZ, A ;

MAQUEDA, M ;

VALDIVIA, E ;

QUESADA, A ;

MONTOYA, E .

CANADIAN JOURNAL OF MICROBIOLOGY, 1986, 32 (10) :765-771

[6] BACTERICIDAL ACTION OF PEPTIDE ANTIBIOTIC AS-48 AGAINST ESCHERICHIA-COLI K-12 [J].

GALVEZ, A ;

VALDIVIA, E ;

MARTINEZ, M ;

MAQUEDA, M .

CANADIAN JOURNAL OF MICROBIOLOGY, 1989, 35 (02) :318-321

[7]

GALVEZ A, 1986, MICROBIOS, V43, P223

[8] MODIFICATION OF STREPTOCOCCUS-FAECALIS SEX-PHEROMONES AFTER ACQUISITION OF PLASMID DNA [J].

IKE, Y ;

CRAIG, RA ;

WHITE, BA ;

YAGI, Y ;

CLEWELL, DB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (17) :5369-5373

[9] MULTIPLE PLASMID-CONTAINING ESCHERICHIA-COLI STRAIN - CONVENIENT SOURCE OF SIZE REFERENCE PLASMID MOLECULES [J].

MACRINA, FL ;

KOPECKO, DJ ;

JONES, KR ;

AYERS, DJ ;

MCCOWEN, SM .

PLASMID, 1978, 1 (03) :417-420

[10]

Maniatis T., 1982, MOL CLONING

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