EXPRESSION OF MOUSE TYROSINE-HYDROXYLASE IN ESCHERICHIA-COLI

被引：13

作者：

ICHIKAWA, S ^{[1
]}

NASRIN, S ^{[1
]}

NAGATSU, T ^{[1
]}

机构：

[1] NAGOYA UNIV,SCH MED,DEPT BIOCHEM,NAGOYA,AICHI 466,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)90159-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enzymatically active mouse tyrosine hydroxylase (TH) was successfully expressed at a high level in Escherichia coli using a T7 RNA polymerase directed expression system. The specific activity of mouse TH in E.coli cell lysate was 7.5 nmol/mg protein/min. Kinetic characteristics of recombinant TH were examined. Km for tyrosine and (6R)-tetrahydrobiopterin (6R-BH4) cofactor were determined to be 7.2 μM (420 μM 6R-BH4), 19μM ([6R-BH4] < 55 μM, 20 μM tyrosine) and 54 μM ([6R-BH4] > 55 μM, 20 μM tyrosine), respectively. These were in good agreement with previously reported values for this enzyme. © 1991.

引用

页码：664 / 671

页数：8

共 23 条

[1] ISOLATION AND NUCLEOTIDE-SEQUENCE OF A CDNA CLONE ENCODING BOVINE ADRENAL TYROSINE-HYDROXYLASE - COMPARATIVE-ANALYSIS OF TYROSINE-HYDROXYLASE GENE-PRODUCTS [J].

D'MELLO, SR ;

WEISBERG, EP ;

STACHOWIAK, MK ;

TURZAI, LM ;

GIOIO, AE ;

KAPLAN, BB .

JOURNAL OF NEUROSCIENCE RESEARCH, 1988, 19 (04) :440-449

[2] CLONING OF QUAIL TYROSINE-HYDROXYLASE - AMINO-ACID HOMOLOGY WITH OTHER HYDROXYLASES DISCLOSES FUNCTIONAL DOMAINS [J].

FAUQUET, M ;

GRIMA, B ;

LAMOUROUX, A ;

MALLET, J .

JOURNAL OF NEUROCHEMISTRY, 1988, 50 (01) :142-148

[3]

FITZPATRICK PF, 1990, J BIOL CHEM, V265, P2042

[4]

GINNS EI, 1988, J BIOL CHEM, V263, P7406

[5] A SINGLE HUMAN-GENE ENCODING MULTIPLE TYROSINE HYDROXYLASES WITH DIFFERENT PREDICTED FUNCTIONAL-CHARACTERISTICS [J].

GRIMA, B ;

LAMOUROUX, A ;

BONI, C ;

JULIEN, JF ;

JAVOYAGID, F ;

MALLET, J .

NATURE, 1987, 326 (6114) :707-711

[6] COMPLETE CODING SEQUENCE OF RAT TYROSINE-HYDROXYLASE MESSENGER-RNA [J].

GRIMA, B ;

LAMOUROUX, A ;

BLANOT, F ;

BIGUET, NF ;

MALLET, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (02) :617-621

[7] MULTIPLE HUMAN TYROSINE-HYDROXYLASE ENZYMES, GENERATED THROUGH ALTERNATIVE SPLICING, HAVE DIFFERENT SPECIFIC ACTIVITIES IN XENOPUS OOCYTES [J].

HORELLOU, P ;

LEBOURDELLES, B ;

CLOTHUMBERT, J ;

GUIBERT, B ;

LEVIEL, V ;

MALLET, J .

JOURNAL OF NEUROCHEMISTRY, 1988, 51 (02) :652-655

[8] A SINGLE RNA SPECIES INJECTED IN XENOPUS OOCYTE DIRECTS THE SYNTHESIS OF ACTIVE TYROSINE-HYDROXYLASE [J].

HORELLOU, P ;

GUIBERT, B ;

LEVIEL, V ;

MALLET, J .

FEBS LETTERS, 1986, 205 (01) :6-10

[9] PRIMARY STRUCTURE OF MOUSE TYROSINE-HYDROXYLASE DEDUCED FROM ITS CDNA [J].

ICHIKAWA, S ;

SASAOKA, T ;

NAGATSU, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 176 (03) :1610-1616

[10] ISOLATION OF A NOVEL CDNA CLONE FOR HUMAN TYROSINE-HYDROXYLASE - ALTERNATIVE RNA SPLICING PRODUCES 4 KINDS OF MESSENGER-RNA FROM A SINGLE GENE [J].

KANEDA, N ;

KOBAYASHI, K ;

ICHINOSE, H ;

KISHI, F ;

NAKAZAWA, A ;

KUROSAWA, Y ;

FUJITA, K ;

NAGATSU, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 146 (03) :971-975

← 1 2 3 →