HIGH-FREQUENCY SOMATIC EMBRYOGENESIS AND PLANT-REGENERATION FROM ZYGOTIC EMBRYO-DERIVED CALLUS-CULTURES OF 3 ALLIUM SPECIES

被引：44

作者：

VANDERVALK, P ^{[1
]}

SCHOLTEN, OE ^{[1
]}

VERSTAPPEN, F ^{[1
]}

JANSEN, RC ^{[1
]}

DONS, JJM ^{[1
]}

机构：

[1] DLO, CTR PLANT BREEDING & REPROD RES, POB 16, 6700 AA WAGENINGEN, NETHERLANDS

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1992年 / 30卷 / 03期

关键词：

ABSCISIC ACID; ALLIUM-CEPA; ALLIUM-FISTULOSUM; ALLIUM-PORRUM; MS MEDIUM;

D O I：

10.1007/BF00040020

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The plant regeneration ability of zygotic embryo-derived callus cultures was studied for 12 A. cepa varieties and accessions, two A. fistulosum varieties, one A. fistulosum X A. cepa interspecific hybrid and two A. porrum varieties. Compact embryogenic callus was induced on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid. The embryogenic calluses of all three Allium species were similar in appearance. For all accessions tested plants could be regenerated at a high frequency from this compact callus through somatic embryogenesis, when using kinetin supplemented MS medium (regeneration medium). Addition of abscisic acid to the regeneration medium stimulated the formation of both somatic embryos and shoots for a number of varieties. Concerning shoot regeneration from callus cultures, significant differences existed between genotypes of all accessions except one.

引用

页码：181 / 191

页数：11

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