ANTIINFLAMMATORY ACTIVITY OF POLYGONUM-BISTORTA, GUAIACUM-OFFICINALE AND HAMAMELIS-VIRGINIANA IN RATS

The aqueous ethanolic extracts of Polygonum bistorta L. Polygonaceae, Guaiacum officinale L. Zygophyllaceae and Hamamelis virginiana L. Hamamelidaceae were screened for anti-inflammatory activity. Administered (100 and 200 mg kg(-1), p.o.) before the induction of carrageenan rat paw oedema, extracts of P. bistorta significantly suppressed both the maximal oedema response and the total oedema response (monitored as area under the time course curve). H. virginiana was inactive and G. officinale was only active at 200 mg kg(-1). At 200 mg kg(-1) administered before the induction of adjuvant arthritis, P. bistorta significantly inhibited both the acute and chronic phases of the adjuvant-induced rat paw swelling, while G. officinale and H. virginiana were only active against the chronic phase. Further studies on P. bistorta (100-800 mg kg(-1)) revealed a dose-dependent inhibition of the carrageenan-induced rat paw oedema over the dose range 100-400 mg kg(-1), the E50 value being approximately 158 5 mg kg(-1). The extract (200 mg kg(-1)), administered after the onset of the inflammatory responses reversed the course of both the carrageenan- and adjuvant- induced rat paw swelling. The results confirm that the extracts of P. bistolta, G. officinale and H. virginiana contain anti-inflammatory substances.