THERMOINACTIVATION OF CELLOBIOHYDROLASE-I FROM TRICHODERMA-REESEI QM-9414

被引：14

作者：

JIMENEZ, J ^{[1
]}

DOMINGUEZ, JM ^{[1
]}

CASTILLON, MP ^{[1
]}

ACEBAL, C ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC CIENCIAS BIOL,DEPT BIOQUIM & BIOL MOLEC 1,E-28040 MADRID,SPAIN

来源：

CARBOHYDRATE RESEARCH | 1995年 / 268卷 / 02期

关键词：

CELLULASE; CELLOBIOHYDROLASE I; THERMAL INACTIVATION; TRICHODERMA REESEI;

D O I：

10.1016/0008-6215(94)00337-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Irreversible thermoinactivation of cellobiohydrolase I from Trichoderma reesei has been analyzed at 70 degrees C and pH 4.8. The time course of thermal inactivation and the dependence of the inactivation rates on protein concentration suggested that aggregation followed by precipitation was the main process leading to irreversible thermoinactivation. The enzyme activity was very resistant to 4 M urea which stabilized the enzyme against thermal inactivation. Deamidation of Asn/Gln residues and hydrolysis of peptide bonds were responsible for the loss of enzyme activity at long times of exposure at 70 degrees C.

引用

页码：257 / 266

页数：10

共 25 条

[1]

ACEBAL C, 1986, APPL MICROBIOL BIOT, V24, P218, DOI 10.1007/BF00261540

[2] THE MECHANISM OF IRREVERSIBLE ENZYME INACTIVATION AT 100-DEGREES-C [J].

AHERN, TJ ;

KLIBANOV, AM .

SCIENCE, 1985, 228 (4705) :1280-1284

[3]

AHERN TJ, 1992, CHEM PHYSICAL PROPER, P215

[4] THE DISULFIDE BRIDGES IN A CELLOBIOHYDROLASE AND AN ENDOGLUCANASE FROM TRICHODERMA-REESEI [J].

BHIKHABHAI, R ;

PETTERSSON, G .

BIOCHEMICAL JOURNAL, 1984, 222 (03) :729-736

[5] INCLUSION-BODIES OF THE THERMOPHILIC ENDOGLUCANASE-D FROM CLOSTRIDIUM-THERMOCELLUM ARE MADE OF NATIVE ENZYME THAT RESISTS 8-M UREA [J].

CHAFFOTTE, AF ;

GUILLOU, Y ;

GOLDBERG, ME .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 205 (01) :369-373

[6] HIGH-RESOLUTION ISOELECTRIC-FOCUSING OF IMMUNOPRECIPITATED PROTEINS UNDER DENATURING CONDITIONS - A SIMPLE ANALYTICAL METHOD APPLIED TO THE STUDY OF COMPLEMENT COMPONENT POLYMORPHISMS [J].

DECORDOBA, SR ;

RUBINSTEIN, P ;

FERREIRA, A .

JOURNAL OF IMMUNOLOGICAL METHODS, 1984, 69 (02) :165-172

[7] AN ASSAY FOR SELECTIVE DETERMINATION OF EXO-1,4,-BETA-GLUCANASES IN A MIXTURE OF CELLULOLYTIC ENZYMES [J].

DESHPANDE, MV ;

ERIKSSON, KE ;

PETTERSSON, LG .

ANALYTICAL BIOCHEMISTRY, 1984, 138 (02) :481-487

[8]

DIXON WJ, 1981, BMDP STATISTICAL SOF

[9] MECHANISMS OF THERMOINACTIVATION OF ENDOGLUCANASE-I FROM TRICHODERMA-REESEI QM-9414 [J].

DOMINGUEZ, JM ;

ACEBAL, C ;

JIMENEZ, J ;

DELAMATA, I ;

MACARRON, R ;

CASTILLON, MP .

BIOCHEMICAL JOURNAL, 1992, 287 :583-588

[10] TISSUE SULFHYDRYL GROUPS [J].

ELLMAN, GL .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 82 (01) :70-77

← 1 2 3 →