ACIDIC PH RAPIDLY INCREASES IMMUNOREACTIVITY OF GLIAL FIBRILLARY ACIDIC PROTEIN IN CULTURED ASTROCYTES

Neuroepithelial progenitor cells from forebrains of newborn rat pups develop into ''mature'' astrocytes in an epidermal growth factor-containing medium free of serum (Von Visger et al: Exp Neurol 128:34, 1994). Eight-week-old ''mature'' astrocyte cultures on poly-l-lysine-coated dishes were exposed to an acidic medium (pH 5.8-6.0) for 2-6 h. Immunoreactivity for glial fibrillary acidic protein (GFAP) dramatically and rapidly increased; this immediate increase was not affected by pretreatment with cyclo-heximide. In further experiments we found that the increase in GFAP was undiminished for 24-48 h after the acid-treated astrocytes were returned to normal growth medium. The Ca2+ channel antagonists nifedipine and diltiazem attenuated the increase in GFAP immunoreactivity. These results suggest that extracellular acidosis may produce a rapid increase in GFAP immunoreactivity in astrocytes independent of de novo protein synthesis, possibly by increasing intracellular levels of free Ca2+ ions. (C) 1995 Wiley-Liss, Inc.