MICRODETERMINATION OF DRUG INGREDIENTS 2-CHLORO-4-NITROBENZAMIDE 4'-[(P-NITROPHENYL)SULFAMOYL]-ACETANILIDE AND 4-AMINOBENZENEARSONIC ACID IN MEDICATED FINISHED FEEDS

Although more than one method is available for determination of the individual drug ingredients, 2-chloro-4-nitrobenzamide (aklomide), 4′-[(p-nitrophenyl) sulfamoyl]acetanilide (sulfanitran), and 4- aminobenzenearsonic acid (p-arsanilic acid), no suitable procedure is reported for their determination when present as a mixture in medicated finished feeds. In this report the estimation of microamounts of aklomide, sulfanitran, and p-arsanilic acid is described. Using a thin-layer alumina chromatoplate, aklomide and sulfanitran were separated from p-arsanilic acid and individually estimated colorimetrically using the Bratton-Marshall reagent. By repeating the determination after alkaline digestion of a separate sample of the same feed, the total of sulfanitran and p-arsanilic acid was obtained, from which p-arsanilic acid was calculated by difference. The recovery of aklomide and sulfanitran was more than 96% and that of p-arsanilic acid was about 90%. © 1969, American Chemical Society. All rights reserved.