ISOLATION AND RECOGNITION PROPERTIES OF SOME DEFINITE SPONGE CELL-TYPES

被引:19
作者
DESUTTER, D
VANDEVYVER, G
机构
[1] Université libre de Bruxelles, Laboratoire de Biologie animale et cellulaire, 1050 Bruxelles, 50, av. F.D. Roosevelt
关键词
D O I
10.1016/S0145-305X(79)80036-1
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Two cell fractions can be isolated from strains of the fresh-water sponges Ephydatia fluviatilis by means of Ficoll gradients. The densest fraction consists of pure archaeocytes; the least dense is very rich in choanocytes and free from archaeocytes. Both fractions aggregate; but archaeocyte aggregates undergo a complete process of reconstitution whereas choanocyte aggregates fail to settle. Both were tested for their allogeneic recognition properties by mixing 3 different proportions of homologous cell fractions isolated from incompatible strains. In equicellular ratios, dominance of one strain or survival of both depending on the paired strain, whereas in unequal ratios, dominance of the most concentrated strain appears to be the rule. Phagocytosis in the aggregates, is enhanced in allogeneic combinations compared to unmixed control samples. © 1979 Pergamon Press Ltd. All rights reserved.
引用
收藏
页码:389 / 397
页数:9
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