MUTATIONS IN THE REGIONS OF THE ROUS-SARCOMA VIRUS 3' SPLICE SITES - IMPLICATIONS FOR REGULATION OF ALTERNATIVE SPLICING

被引:32
作者
BERBERICH, SL
STOLTZFUS, CM
机构
[1] UNIV IOWA, DEPT MICROBIOL, IOWA CITY, IA 52242 USA
[2] UNIV IOWA, GENET PROGRAM, IOWA CITY, IA 52242 USA
关键词
D O I
10.1128/JVI.65.5.2640-2646.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Retrovirus RNA is synthesized as a single primary transcript that is differentially processed by RNA splicing. Three species of viral RNA (spliced env. spliced src, and unspliced full-length RNA) are produced in chicken embryo fibroblasts infected with Rous sarcoma virus, an avian retrovirus. The env and src mRNAs are synthesized by the alternative use of two 3' splice sites. The mechanism by which balanced splicing at the two sites is maintained was investigated in this report. Mutants that increase or decrease splicing at one of the two 3' splices sites were analyzed for the effect on splicing at the other site. The two splice sites differed in their response. Mutations that caused a specific increase in the level of spliced env mRNA were associated with reciprocal changes in the levels of src mRNA but with no change in overall splicing. In contrast, mutants in which the src 3' splice site was inactivated demonstrated a decreased overall level of spliced RNA but had little or no effect on the level of spliced env mRNA. Mutations that caused specific increases in the level of spliced src mRNA had variable effects on env mRNA levels. Deletion of regions in gag, which was previously shown to contain a cis-acting negative regulator of splicing, resulted in a corresponding increase of both spliced viral mRNAs and a decrease in unspliced RNA, suggesting that this element suppressed both env and src splicing. Several models are discussed which are possible mechanisms for regulation of alternative splicing of Rous sarcoma virus RNA, but none of these models appear to be consistent with all of the data.
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页码:2640 / 2646
页数:7
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