LIPID ARRANGEMENT IN FLUID MODEL MEMBRANES - ANALYSIS BY CROSS-LINKING OF PHOSPHATIDYLETHANOLAMINES

被引：10

作者：

ROTH, MR ^{[1
]}

WELTI, R ^{[1
]}

机构：

[1] KANSAS STATE UNIV AGR & APPL SCI, DIV BIOL, ACKERT HALL, MANHATTAN, KS 66506 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1063卷 / 02期

关键词：

PHOSPHOLIPID PHASE TRANSITION; PHOSPHATIDYLETHANOLAMINE; FLUID-FLUID IMMISCIBILITY; CROSS-LINKING; DIMETHYL SUBERIMIDATE;

D O I：

10.1016/0005-2736(91)90377-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Binary mixtures of fluid phase phosphatidylethanolamines at pH 10 were treated with the bifunctional cross-linking reagent dimethylsuberimidate. Analysis of the dimeric species formed demonstrated that the phospholipid species in dimyristoylphosphatidylethanolamine / dielaidoylphosphatidylethanolamine mixtures at 52-degrees-C and dielaidoylphosphatidylethanolamine / dilauroylphosphatidylethanolamine mixtures at 41-degrees-C were randomly arranged. Analysis of the dimeric species formed in dipalmitoylphosphatidylethanolamine / dioleoylphosphatidylethanolamine mixtures at 68-degrees-C showed that this mixture was very close to being randomly arranged, with just a slight propensity of like phospholipid species to cluster.

引用

页码：242 / 246

页数：5

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