CELL-GROWTH EXPERIMENTS USING A MICROCALORIMETRIC VESSEL EQUIPPED WITH OXYGEN AND PH ELECTRODES

被引：44

作者：

BACKMAN, P ^{[1
]}

WADSO, I ^{[1
]}

机构：

[1] UNIV LUND,CTR CHEM,DIV THERMOCHEM,POB 124,S-22100 LUND,SWEDEN

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1991年 / 23卷 / 04期

关键词：

MICROCALORIMETRY; POLAROGRAPHIC OXYGEN SENSOR; PH-ELECTRODE; T-LYMPHOMA CELL; ESCHERICHIA-COLI;

D O I：

10.1016/0165-022X(91)90003-F

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for simultaneous measurements of heat production rate, oxygen activity and pH, using a 3-ml stirred microcalorimetric titration/perfusion vessel fitted with a polarographic oxygen sensor and a combination pH-electrode. Heat production rate (+/- 0.3-mu-W), pH (+/- 0.01) and oxygen concentration (+/- 4-mu-mol/l) could be measured without any cross interference between the three signals. Results from measurements on suspensions of T-lymphoma cells and E. coli.are presented.

引用

页码：283 / 293

页数：11

共 26 条

[1] EFFECTS OF EXPERIMENTAL FACTORS ON THE METABOLIC-RATE OF T-LYMPHOMA CELLS AS MEASURED BY MICROCALORIMETRY [J].