SHEEP RED-BLOOD-CELL INSTILLATION AT PALATINE TONSIL EFFECTIVELY INDUCES SPECIFIC IGA CLASS ANTIBODY IN SALIVA IN RABBITS

被引:22
作者
FUKUIZUMI, T
INOUE, H
ANZAI, Y
TSUJISAWA, T
UCHIYAMA, C
机构
[1] Department of Oral Bacteriology, Kyushu Dental College, Fukuoka 803, Manazuru 2-6-1, Kokura-kita-ku, Kitakyushu
关键词
PALATINE TONSIL; SALIVA; SECRETORY IGA; MUCOSAL IMMUNITY;
D O I
10.1111/j.1348-0421.1995.tb02212.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have shown that the palatine tonsil effectively incorporates exogenous foreign substances instilled at its surface, It is not clear whether antigen-specific IgA can be induced by the instillation. Sheep red blood cells (SRBC) were instilled at the palatine tonsil every three days as the antigen, and the agglutination titer of specific IgA in saliva was examined. Nasal or intragastric administration, which have been shown to induce specific antibody in saliva, were done as control experiments, Anti-SRBC antibody in saliva from the tonsillar instillation group was detected in the second week, and the agglutination titer reached a maximum in the 6th week after the instillation. The maximum titers in the tonsillar instillation group and nasal administration group were 16 (P<0.01, n=7) and 4 times (P<0.01, n=7) higher, respectively, than that in the intragastric administration group. In the tonsillar instillation group, the number of specific antibody-producing cells per 10(5) lymphocytes was the highest in the parotid glands compared with the lymphoid tissues such as the retropharyngeal lymph nodes, nasal mucosa, mesenteric lymph nodes, Peyer's patches, cervical lymph nodes, palatine tonsil and spleen. In the nasal administration group, the number of lymphocytes was the highest in the nasal mucosa. The results indicate that tonsillar instillation was more effective than nasal administration in inducing specific IgA in saliva.
引用
收藏
页码:351 / 359
页数:9
相关论文
共 32 条
  • [1] Ali M.Y., Histology of the human nasopharyngeal mucosa, J. Anat, 99, pp. 657-672, (1965)
  • [2] Bhalla D.K., Murakami T., Owen R.L., Microcirculation of intestinal lymphoid follicles in rat Peyer’s patches, Gastroenterology, 81, pp. 481-491, (1981)
  • [3] Brandtzaeg P., Mucosal glandular distribution of immunoglobulin components. Differential localization of free and bound SC in secretory epithelial cells, J. Immunol, 112, pp. 1553-1559, (1974)
  • [4] Brandtzaeg P., Immune functions of human nasal mucosa and tonsils in health and disease, pp. 28-95, (1984)
  • [5] Brandtzaeg P., Distribution and characteristics of mucosal immunoglobulin-producing cells, pp. 251-262, (1994)
  • [6] Couch R.B., Kasai J.A., Immunity to influenza in man, Ann. Rev. Microbiol, 37, pp. 529-549, (1983)
  • [7] Czerkinsky C., Russel M.W., Lycke N., Lindblad M., Holmgren J., Oral administration of a streptococcal antigen coupled to cholera toxin B subunit evokes strong antibody responses in salivary glands and extramucosal tissues, Infect. Immun, 57, 4, pp. 1072-1077, (1989)
  • [8] Fukuizumi T., Inoue H., Anzai Y., Hase K., Uchiyama C., Incorporation of instilled Candida albicans and lipopolysaccharide into the palatine tonsil of rabbit, Jpn. J. Oral Biol, 36, 3, pp. 222-229, (1994)
  • [9] Hirabayashi Y., Kurata H., Funato H., Nagamine T., Aizawa C., Tamura S., Shimada K., Kurata T., Comparison of intestinal inoculation of influenza HA vaccine combined with cholera toxin B subunit with oral or parenteral vaccination, Vaccine, 8, pp. 243-248, (1990)
  • [10] Kiyono H., Bienenstok J., McGhee J.R., Ernst P.B., The mucosal immune system: features of inductive and effector sites to consider in mucosal immunization and vaccine development, Reg. Immunol, 4, 2, pp. 54-62, (1992)