INDUCTION OF PROTEIN-SYNTHESIS IN RESPONSE TO COLD SHOCK IN THE PSYCHOTROPIC YEAST TRICHOSPORON-PULLULANS

The synthesis of proteins in response to a cold shock in the psychrotrophic yeast Trichosporon pullulans was examined. Two-dimensional electrophoretic analysis of a 21 to 5° C cold shock revealed the induction of 26 cold-shock proteins (csps) at the maximum induction time of 12 h. The induction of only 10 csps after a 24 to 5°C cold shock suggested that the growth of cells above their optimum growth temperature reduced their ability to induce csps. Comparison of the csp profile obtained after a 15 to a 5°C cold shock with the profile for the 21 to 5°C cold shock suggested that the range of the cold shock influences which proteins are induced.