HORMONE-RECEPTOR INTERACTIONS - STUDY OF THE BINDING OF HORMONE-SUBSTITUTED TOBACCO MOSAIC-VIRUS TO MEMBRANE-VESICLES BY DYNAMIC LIGHT-SCATTERING AND BY TRANSIENT ELECTRIC BIREFRINGENCE

被引：11

作者：

SCHWYZER, R ^{[1
]}

KRIWACZEK, VM ^{[1
]}

BAUMANN, K ^{[1
]}

HALLER, HR ^{[1
]}

WIDER, G ^{[1
]}

WILTZIUS, P ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL, FESTKORPERPHYS LAB, CH-8093 ZURICH, SWITZERLAND

来源：

PURE AND APPLIED CHEMISTRY | 1979年 / 51卷 / 04期

关键词：

D O I：

10.1351/pac197951040831

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Tobacco mosaic virus has been covalently conjugated with 500 - 600 molecules of different hormones. The particles react with antisera against the hormones and with hormone receptors in target organs, on target cells, and on membrane vesicles. The compounds were used for cooperative affinity labelling and for the isolation of receptor-bearing vesicles. The binding of a tobacco mosaic virus/angiotensin II conjugate to adrenal cortex membrane vesicles was studied by dynamic light scattering and transient electric birefringence. Despite the complexity of the polydisperse systems and the fact that the membrane vesicles exhibit a Kerr effect and depolarize the light, methods were developed that allowed the observation and theoretical treatment of the effects due to specific binding. © IUPAC

引用

页码：831 / 835

页数：5

共 10 条

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[10]

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