FREE-RADICAL SCAVENGERS AMELIORATE THE 2-CELL BLOCK IN MOUSE EMBRYO CULTURE

被引：111

作者：

LEGGE, M ^{[1
]}

SELLENS, MH ^{[1
]}

机构：

[1] UNIV ESSEX,DEPT BIOL,WIVENHOE PK,COLCHESTER CO4 3SQ,ESSEX,ENGLAND

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 06期

关键词：

FREE RADICALS; EMBRYO CULTURE; 2-CELL BLOCK;

D O I：

10.1093/oxfordjournals.humrep.a137442

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Mouse zygotes from a strain combination which fails to undergo cleavage in vitro (the 2-cell block) were cultured in a variety of media, using either low (5%) or normal (20%) oxygen tension in the gas phase. Development beyond the 4-cell stage was only achieved in medium containing serum and with normal oxygen tension. When serum-free medium was supplemented with free radical scavengers, reduced glutathione (1 mM), but not histidine, catalase or superoxide dismutase, development to the morula or blastocyst stage was promoted in 50% of zygotes cultured with normal oxygen tension. These data suggest that the 2-cell block is a consequence, at least in part, of free radical damage incurred by embryos during collection and culture, and that medium supplementation with the radical scavenger, reduced glutathione, can improve embryo development in vitro.

引用

页码：867 / 871

页数：5

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