GENOMIC ORGANIZATION OF LIGNIN PEROXIDASE GENES OF PHANEROCHAETE-CHRYSOSPORIUM

被引：36

作者：

GASKELL, J ^{[1
]}

DIEPERINK, E ^{[1
]}

CULLEN, D ^{[1
]}

机构：

[1] INST MICROBIAL & BIOCHEM TECHNOL,FOREST PROD LAB,1 GIFFORD PINCHOT DR,MADISON,WI 53705

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 03期

关键词：

D O I：

10.1093/nar/19.3.599

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three lignin peroxidase (LiP) genes from the basidiomycete Phanerochaete chrysosporium were cloned on a single 30 kb cosmid insert. One gene, GLG5, is the genomic equivalent of a previously reported cDNA clone, CLG5. The other two LiP genes are transcriptionally convergent and map to a position approximately 15 kb downstream of GLG5. The translational stop codons of these genes are separated by 1.3 kb. Analysis of homokaryons established allelic relationships to previously described LiP clones. Using clamped homogeneous electrical field electrophoresis (CHEF), seven chromosomal bands were resolved from P. chrysosporium genomic DNA. On CHEF gel Southern blots, the LiP gene family was localized to a single, dimorphic chromosome.

引用

页码：599 / 603

页数：5

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