GLUCOSE BIOSENSOR USING GLUCOSE-OXIDASE IMMOBILIZED IN POLYANILINE

被引：52

作者：

PARENTE, AH ^{[1
]}

MARQUES, ETA ^{[1
]}

AZEVEDO, WM ^{[1
]}

DINIZ, FB ^{[1
]}

MELO, EHM ^{[1
]}

FILHO, JLL ^{[1
]}

机构：

[1] UNIV FED PERNAMBUCO,DEPT QUIM FUNDAMENTAL,BR-50730 RECIFE,PE,BRAZIL

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1992年 / 37卷 / 03期

关键词：

GLUCOSE OXIDASE; POLYANILINE; BIOSENSOR; OXYGEN ELECTRODE;

D O I：

10.1007/BF02788878

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A biosensor for glucose utilizing kinetics of glucose oxidase (EC 1.1.3.4.) was developed. The enzyme was immobilized on polyaniline by covalent bonding, using glutaraldehyde as a bifunctional agent. The system showed a linear response up to 2.2 mM of glucose with a response time of 2.5-4.0 min. In addition, the immobilized enzyme had a higher activity between pH 6.5 and 7.5. The system retained 50% of its activity after 30 d of daily use. The optical absorption spectra of the polyaniline/glucose oxidase electrode after glucose had been added to the buffer solution showed that the absorption band around 800 nm had changed considerably when glucose was allowed to react with the electrode. This optical variation makes polyaniline a very promising polymer for use as a support in optical sensor for clinical application.

引用

页码：267 / 273

页数：7

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